Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
نویسندگان
چکیده
منابع مشابه
A new set of allele-specific PCR primers for identification of true hybrids in normal oleate × high oleate crosses in groundnut
Allele-specific PCR (AS-PCR) with 3 primers was used to identify true F1 hybrids resulting from normal oleate groundnut cultivars pollinated with high oleate groundnut genotypes with a 1-bp insertion (A:T) at position 442 after the start codon in FAD2B. Its reliability was confirmed by direct sequencing of FAD2B PCR products. The AS-PCR protocol requires minimal resources, and thus provides an ...
متن کاملPartial Dominance , Pleiotropism , and Epistasis in the Inheritance of the High - Oleate Trait in Peanut
Earlier reports of the high-oleate (low-linoleate) trait in peanut (Arachis hypogaea L.) indicated that it was controlled by completely dominant gene action. However, linoleate content intermediate to that in lowand normal-linoleate seeds was found among progeny when the trait was backcrossed into five virginia-type cultivars, suggesting partial dominance of the gene controlling the trait. Alth...
متن کاملIdentification of a novel mutation in FAD2B from a peanut EMS mutant with elevated oleate content.
A Virginia type peanut mutant with more than 60% oleate content (E2-4-83-12) was selected from an EMS mutagenized population of LF2 (an export type peanut cultivar with 44.2% oleate) by near infrared reflectance spectroscopy. Cloning and sequencing of FAD2B from LF2 and E2-4-83-12 identified a novel mutation (C313T in the coding region) causing an H105Y substitution in the first histidine box o...
متن کاملConformation of the oleate chains in crystals of cholesteryl oleate at 123 K.
At 123 K, crystals of cholesteryl cis-9-octadecenoate (cholesteryl oleate, C45H78O2) are monoclinic, space group P2(1) with unit cell dimensions a = 12.356(2), b = 8.980(3), c = 18.382(2) A, beta = 85.49(2) degrees, and have two molecules in the unit cell. The crystal structure including all H atoms has been determined from 3812 independent X-ray reflections with sin theta/lambda less than 0.61...
متن کاملEarly oleate deficiency leads to severe defects in fetal rat liver development
Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat ...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2010
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol13-issue5-fulltext-18